Molecules, DNA and Protein

These tools start from a formula, sequence or bench reading. Get molar mass from a formula, convert mass to moles, balance an equation and find the limiting reagent, find protein molecular weight, DNA or RNA concentration and molarity, ligation insert amounts and protein concentration.

Tools in this group

• DNA Concentration Calculator (A260) — Enter the A260 reading and the dilution to get the DNA or RNA concentration, yield and purity ratios.
• DNA Molarity Calculator — Enter the DNA concentration and length to get the molar concentration, pmol and copies per µL.
• Ligation Calculator (Insert : Vector Ratio) — Enter the vector and insert lengths, the vector mass and the molar ratio to get the insert mass and the reaction volumes.
• Calculating Moles: Mass to Moles Calculator — Convert a mass to moles and particles, or moles back to grams, using the molar mass from a formula or a number.
• Molecular Weight Calculator (Molar Mass) — Type a chemical formula such as CuSO4·5H2O to get its molar mass and element composition.
• Protein Concentration Calculator (A280) — Enter the A280 reading and the protein's extinction coefficient, or its sequence, to get mg/mL and µM.
• Calculate Molecular Weight of Protein — Paste a protein sequence or FASTA to get its molecular weight in Da and kDa, its monoisotopic mass and its composition.
• Stoichiometry Calculator — Enter a chemical equation and the amounts you have to find the limiting reagent, theoretical yield and percent yield.